Project #4

Elucidating the underlying mechanisms of cell cycle regulation in limb regeneration

Why only certain animal species can regenerate complex structures remains an important and open question. Technological advances have now opened the door to studying the molecular mechanisms of highly regenerative species, like salamanders. A unique aspect of regeneration in these species is that fully differentiated cells are coaxed to re-enter the cell cycle. This reentry is typically blocked by tumor suppressor genes.

This project is focused on how salamanders can prompt cell cycle reentry, dedifferentiation, and rapid cell proliferation without deleterious effect (i.e., cancer formation). We will develop novel transgenic lines that allow for transient depletion of key tumor suppressor genes. This will allow for the study of tumor suppressor function at homeostasis and during regeneration.